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. Author manuscript; available in PMC: 2014 Jul 1.
Published in final edited form as: Clin Immunol. 2013 Mar 26;148(1):44–55. doi: 10.1016/j.clim.2013.03.008

Figure 7. Supernatants from allogeneic MLR demonstrate XRT enhances TNF-α and IFN-γ cytokine production, but not IL-4.

Figure 7

Unirradiated and irradiated U937 cells were combined with purified CD4+ T cells, in a 1(APC):10(T) ratio and on days 1, 2, 4, and 6, supernatants were used to assay IL-4, TNF-α, and IFN-γ cytokine production via ELISA. Concentrations of cytokines were measured as pg/ml. (A) TNF-α and IFN-γ cytokine production after 0 Gy and 2 Gy. (B) IL-4 cytokine production before and after radiation (2 Gy). (C) Supernatant TNF-α and IFN-γ cytokine concentrations from MLR where U937 cells were pre-treated with control peptide before 0 or 2 Gy). (D) IL-4 cytokine concentration from supernatants of the same MLR as Figure 7C. (E) Shows inhibition of TNF-α and IFN-γ cytokine production where U937 cells were pre-treated with NBD peptide before mock-irradiation or irradiation and combining with CD4+ T cells. (F) Unchanged IL-4 cytokine production from supernatants of the same MLR as Figure 7E . (A-F) Shown is the average cytokine production (from triplicates) ±S.E.M. from three independent experiments. **(P=<0.01) and ****(p=<0.0001).