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. 2014 Jun 4;9(6):e98370. doi: 10.1371/journal.pone.0098370

Figure 1. Inhibitory effects of panepoxydone on the proliferation of human breast cancer cells.

Figure 1

MCF-7, MDA-MB-231, MDA-MB-468 and MDA-MB-453 breast cancer cells were grown in 96 well microtitre plates (5000 cells/well) and treated with increasing concentrations of PP or with DMSO (0.2% vehicle control) and analyzed by CellTiter Glow assay. (A) Time-dependent anti-proliferative effect of PP on breast cancer cells. Data are presented as the means ± SD (n = 2). (B) Dose-dependent anti-proliferative effect of PP on breast cancer cells after 72 hrs. Data are presented as the means ± SD (n = 4). PP inhibited cell viability in a dose-dependent manner for all the cell types suggesting anti-cancer activity of PP. (C) Morphological changes of breast cancer cells after treatment with PP. Based on the IC50 value, 3 doses were selected for subsequent experiments: D1 (half of IC50, D2 (IC50) and D3 (2×IC50). Cells (1.5×104 cells/well) were seeded in 6-well plates and incubated with increasing concentrations of PP or DMSO (0.2% vehicle control) for 24 hrs. Morphological changes were observed under the inverted phase-contrast microscope and photographed. Representative micrographs are from one of the random fields of view (magnification 200X) of cells.