FIG. 2.
Effect of anti-human TLR4 MAb in cytokine production and cytotoxic activity induced by AILb-A. PBMCs were stimulated with AILb-A (1 μg/ml) or LPS (1 μg/ml) in the presence or absence of specific MAbs against human TLR4 (HTA125; 10 μg/ml) or against human CD14, a coreceptor for LPS (10 μg/ml) for 24 h. Then, the supernatants were analyzed for TNF-α (A) and IL-12 (B) by ELISA. Cytotoxic activity against K-562 of the PBMCs was assayed by 51Cr release test (C). Bars denote standard deviations of triplicate samples, *, P < 0.01.