FIG. 3.

Impairment of cytokine-inducing activity of AILb-A in PMs derived from TLR4^−/− and TLR4 mutant mice. Thioglycolate-elicited PMs (5 × 10⁵/ml) derived from wild-type C57BL/6, TLR2^−/−, TLR4^−/−, C3H/HeN, and C3H/HeJ mice were treated with AILb-A (0.1, 1, or 10 μg/ml), PGN (2 μg/ml), or LPS (1 μg/ml) for 48 h, and then TNF-α (A) and IL-12 (B) in the supernatants were measured by ELISA. Bars denote standard deviations of triplicate samples, *, P < 0.01 compared with untreated controls.