FIG. 7.

Involvement of TLR4 in AILb-A-induced phosphorylation of p38 MAPK and JNK. PMs (5 × 10⁵/ml) were stimulated with AILb-A (1 μg/ml), LPS (1 μg/ml), or PGN (2 μg/ml) for 1 h and then lysed in 1× SDS sample buffer. The samples (30 μg of protein/lane) were electrophoresed on SDS-10% polyacrylamide gels, transferred onto polyvinylidene difluoride membranes, and immunoblotted with an Ab specific for phospho-p38 MAPK (A, upper panel) or phospho-JNK (B, upper panel). The same blots were stripped and reblotted with an Ab specific both for phosphorylated and for nonphosphorylated forms of each respective signaling protein (A and B, lower panels). These are representative blots from three independent experiments.