Figure 2.

P-sel distribution in megakaryocytes of wild type and Gata1^low mice. Semi thin sections of spleen from WT (a) and Gata1^low (b) mice, immune stained for P-sel and analyzed by immuno-electron microscopy. Representative MKs are presented on the left panels and the region of their cytoplasm included in the rectangle are shown at greater magnification in the panels on the right, P-sel staining appears as dots. P-sel was found in the α-granule membrane (arrow heads) as well as in the cytoplasm of MKs from wild type mice. However, in the immature MKs from Gata1^low mice, P-sel staining was found primarily in the cytoplasm, lining the DMS (arrow-heads).