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. Author manuscript; available in PMC: 2015 Mar 21.
Published in final edited form as: Chembiochem. 2014 Mar 11;15(5):721–733. doi: 10.1002/cbic.201300797

Figure 2.

Figure 2

LC-MS data (total ion current vs. time) of crude DARPin fragments. The initial as well as the final syntheses are shown for every peptide. Each panel also displays peptide sequences, MS insets of the major peak, comparison of monoisotopic calculated and observed molecular masses for the expected product, and a table with identification of the major impurities. The charge state series on the inset mass spectra display the most abundant ions; observed and calculated masses are monoisotopic. The data for a) DARPin fragment 1; b) DARPin fragment 2; c) DARPin fragment 3; cysteine highlighted in grey was incorporated as Cys(Acm); d) DARPin fragment 4.