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. Author manuscript; available in PMC: 2015 Mar 21.
Published in final edited form as: Chembiochem. 2014 Mar 11;15(5):721–733. doi: 10.1002/cbic.201300797

Figure 5.

Figure 5

LC-MS data (total ion current vs time) of purified Barnase ligation products. Each panel also displays MS insets of the major peak, comparison of average calculated and observed molecular masses for the expected product, and a deconvolution result. The charge state series on the inset mass spectra display the most abundant ions; observed and calculated masses are average. a) Purified N-terminal polypeptide H2N-[1Gly-39Val]-CONHNH2. b) Purified C-terminal polypeptide H2N-[40Cys-113Arg]-CONH2. c) Purified full length Barnase [14,40,77Cys]. d) Purified full length, native, desulfurized Barnase.