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Characterization of the synthesized proteins. a) CD spectrum of the synthesized DARPin pE59. b) CD spectrum of the synthesized Barnase. c) SPR binding assay showing the synthetic DARPin pE59 binding to pERK2; KD of the complex was found to be 190 nM. d) Barnase RNase activity assay; specific activity was calculated using additional data points (see the SI).
