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. 2004 May;11(3):458–462. doi: 10.1128/CDLI.11.3.458-462.2004

TABLE 1.

Assay results for experimentally vaccinated dogsa

Vaccine Sample time SNAP 3Dx result IFA titer, dog 1, 2, 3 WB result, (molecular mass[es] of reactive band[s] [kDa]) C6 peptide microtiter plate ELISA result (mean ODb)
LymeVax (Fort Dodge-Animal Health) Prebleed Neg All < 100 Neg 0.053
Wk 5 Neg 1,600, 1,600, 800 41, 34, 31, 18 0.060
Wk 33 Neg 400, 400, 100 41, 39, 34, 31, 21, 18 0.061
Wk 34 Neg 1,600, 1,600, 1,600 93, 66, 60, 58, 41, 39, 34, 31, 21, 18 0.062
Wk 40 Neg 6,400, 1,600, 1,600 93, 66, 60, 58, 41, 39, 34, 31, 30, 28, 23, 21, 18 0.073
Wk 43 Neg 3,200, 1,600, 1,600 Not tested 0.069
Galaxy (Schering-Plough Animal Health) Prebleed Neg All < 100 Neg 0.043
Wk 5 Neg 1,600, 1,600, 1,600 66, 58, 41, 39, 34, 31, 21, 18 0.079
Wk 33 Neg 400, 100, 100 66, 58, 39, 34, 31, 21 0.072
Wk 34 Neg 3,200, 1,600, 1,600 93, 66, 60, 58, 41, 39, 34, 31, 30, 23, 21, 18 0.069
Wk 40 Neg 6,400, 6,400, 3,200 93, 66, 60, 58, 41, 39, 34, 31, 30, 23, 21, 18 0.078
Wk 43 Neg 3,200, 3,200, 3,200 Not tested 0.072
RECOMBITEK Lyme (Merial) Prebleed Neg All < 100 41 0.043
Wk 5 Neg 800, 800, 800 41, 31 0.087
Wk 33 Neg 100, 100, 100 41, 31 0.058
Wk 34 Neg 1,600, 1,600, 800 93, 66, 41, 31, 18 0.060
Wk 40 Neg 3,200, 3,200, 3,200 93, 66, 41, 31, 18 0.064
Wk 43 Neg 1,600, 1,600, 800 Not tested 0.065
None (control) Prebleed Neg All < 100 Neg 0.055
Wk 5 Neg All < 100 Neg 0.054
Wk 33 Neg All < 100 Neg 0.066
Wk 34 Neg All < 100 Neg 0.055
Wk 40 Neg All < 100 Neg 0.053
Wk 43 Neg All < 100 Not tested 0.067
a

Neg, negative.

b

OD, optical density at 650 nm. Assay cutoff, 0.097.