Table 1. Characterization of the coupled genes for Pik-h via gain and loss of function approaches.
| Recipient | Inoculation | Reaction of T0 Plants a | Success | |||||
| Candidate/construct | Cultivar | Isolate | R | MR | MS | S | Total | ratio (%) b |
| Gain of function | ||||||||
| Pikh-1 | Q1063 | CHL346 | 0 | 0 | 1 | 149 | 150 | 0 |
| Pikh-2 | Q1063 | CHL346 | 0 | 0 | 0 | 82 | 82 | 0 |
| Pikh-12 | Kuyuku 131 | CHL42 | 7 | 3 | 1 | 8 | 19 | 42.1 |
| Pikh-12 | K60 | CHL42 | 82 | 14 | 14 | 4 | 114 | 84.2 |
| Loss of function c | ||||||||
| Pikh-1 RNAi (KP3i1) | K3 | CHL346 | 21 | 0 | 4 | 17 | 42 | 50.0 |
| Pikh-2 RNAi (KP4i) | K3 | CHL346 | 5 | 0 | 0 | 20 | 25 | 80.0 |
a
T0 plants derived from each construct were inoculated with the Pikh-avirulent isolates, CHL346 or CHL42. R, resistant; MR, moderate resistant; MS, moderate susceptible; S, susceptible.
b
The success ratios for gain- and loss-of-function complementation tests were calculated as (R+MR)/(R+MR+MS+S), and (MS+S)/(R+MR+MS+S), respectively.
c
The Pik allele-specific RNAi constructs, KP3 RNAi1 and KP4 RNAi, which correspond to Pikp-1 and Pikp-2, respectively [40], were transformed into the Pik-h carrier cv K3.