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ICP10ΔPK induces Th1 immunity and is defective in IL-10 production. T cells from lymph nodes of mice (Swiss Webster, 5 weeks old) given ICP10ΔPK or HSV-2 (106 PFU) were cultured with HSV-2 antigen (10 μg/ml) for 2 days, and supernatants were assayed for IFN-γ (Th1 cytokine) and IL-10 (Th2 cytokine) by enzyme-linked immunosorbent assay. Results are expressed as cytokine levels.
