Table 1. Primers used in this study to amplify R2B and F strains of NDV.

Name	Primer sequence (5′–3′)	Genomic locationab	Expected size
Strain R2B
1Fc	ACCAAACAGAGAATCCGTGAA	1–21	1900 bp
1Rd	GTGAAGGTGGCCATCTTCACT	1880–1900
2F	ACAACACGGGCACAACTCGAC	1741–1761	1559 bp
2R	GATGAGTCCATCCTGGCACAA	3280–3300
3F	TTCCATCCCACTGAATGATCG	3191–3211	2171 bp
3R	AGGGTTACCGGTGATCAAGCC	5342–5362
4F	GGCTCTTTACAATCTGGCTGG	5251–5271	2230 bp
4R	GACTTAGCCATCCGAATCTGG	7461–7481
5F	GGTTGCGATTGCGTGAGTCAC	7356–7376	2044 bp
5R	GGTTGCGATTGCGTGAGTCAC	9380–9400
6F	CTAATGGAGGGATTCGCATAC	9242–9262	2046 bp
6R	CCCAATCTCCATTTCCAGGCG	11268–11288
7F	GTGCACTCATATGTCCTGACC	11093–11113	1951 bp
7R	CGTCGAGTGCAAGAGACTAGC	13024–13044
8F	CACCCTGTCATCCATTCAAGG	12923–12943	2263 bp
8R	CCCACCAAACAAAGATTTGGTGAATG	15164–15186
Strain F
1Fc	ACCAAACAGAGAATCCGTAAGTTA	1–24	2363 bp
1Rd	GTTTCCGCGGCTGGGTTGACTCCCCT	2338–2363
2F	AGTCAACCCAGCCGCGGAAACAG	2343–2365	2621 bp
2R	GAGCTGCGGCCGCTGTTATTTG	4943–4964
3F	AACAGCGGCCGCAGCTCTGAT	4948–4968	1353 bp
3R	TACAACGCGTAGTTTTTTCTTAACTC	6276–6301
4F	AACTACGCGTTGTAGATGACCAAAG	6288–6312	2078 bp
4R	GCACTACGTATTTTGCCTTGTATCTC	8351–8366
5F	CAAAATACGTAATGGTAAATAATACGGGTAGGACATG	8358–8383	1694 bp
5R	TCAGCTTAGCGAAGATCCGTCCATTAACT	10024–10052
6F	CTTCGCTAAGCTGACAAAGAAGTTAAGGAACTG	10039–10071	2805 bp
6R	GTCTAGGCCTCTTACTCTCAGGTAATAG	12817–12844
7F	AGAGGCCTAGACAATATTGTCT	12833–12854	2353 bp
7R	ACCAAACAAAGATTTGGTGAATGACGAG	15159–15186

^aGenomic location of strain R2B is based on the complete genomic sequence of NDV strain Mukteswar.

^bGenomic location of strain F is based on the complete genomic sequence of NDV strain La Sota.

^cF stands for forward primer.

^dR stands for reverse primer.