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a. Double reciprocal plots of NADH concentrations versus reaction rates. b. The secondary plots of the slopes for product inhibition of DHA for Mn-GDH. Reaction conditions: DHA (□: 0 mmol/L, Ο: 0.10 mmol/L, Δ: 0.20 mmol/L, ◊: 0.40 mmol/L); glycerol 0.40 mol/L; Mn-GDH, 1 mg/L; pH 12.0; temperature, 45°C.
