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. 2014 Jun 4;9(6):e99162. doi: 10.1371/journal.pone.0099162

Table 1. Kinetic parameters of Mn-GDH and GDH.

Kinetic parameters Mn-GDH GDH
KmA * (mmol/L) 0.008±0.001 0.005±0.001
KmB *(mmol/L) 0.226±0.013 0.262±0.014
KiA (mmol/L) 0. 012±0.002 0.008±0.001

*KmA, the Michaelis-Menten constant of NAD+; KmB, the Michaelis-Menten constant of glycerol.

Reaction conditions: Glycerol concentration (0.010–0.400 mmol/L); enzyme, 1 mg/L; pH 12.0; temperature, 45°C.