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(A) Opening flower bud of amiRchs1 transgenic plants (total RNA was extracted at this stage). (B) V26 (wild-type) flower. (C) amiRchs1 transgenic flower. (D–E) qRT-PCR detection of mRNA levels of the CHS-A (D) and CHS-J (E) gene in V26 and amiRchs1 transgenic petals. Data were normalized against petunia ubiquitin gene and were means of three biological pools (each with three technical replicates); the error bars indicate SD.
