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. 2014 Jun 4;9(6):e98579. doi: 10.1371/journal.pone.0098579

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© 2014 Jankovics et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A,B) Immunostaining of a wild type (A) and a homozygous pbl³ mutant embryo (B). Pbl mutants have fewer germ cells, misguided germ cells and abnormally compacted gonads. Vasa staining labels germ cells (red), Tj staining labels the somatic gonad precursor cells (green). Week Tj expression is also detectable in the cenrtral nervous system. The outline of the embryos is marked by Fas3 staining (blue). Scale bar represents 50 µm. (C–F) Immunofluorescence images of adult gonads. Wild type ovariole (C) and testis (E) contain high number of developing germ cells. Nos-Gal4-VP16/UAS-pbl-shRNA (TRiP.GL01092) ovariole (D) and testis (F) lack germ cells. Vasa staining labels germ cells (red), DAPI labels the nuclei (blue). Anterior is to the left. Scale bar represents 20 µm.