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. 2014 Jun 4;9(6):e98791. doi: 10.1371/journal.pone.0098791

Figure 5. Effect of overexpression of β clamp on growth of AB1157.

Figure 5

Representative images of LB agar plates showing pBR322 (control) or pJRC210 (β+) transformants of strain AB1157 following incubation at (A) 30° or (B) 42°C, as noted. (C) Diameters of representative CFUs shown in panels A and B were measured as described in Materials and Methods. The asterisk (*) indicates strains whose average colony diameter was below the measurement limit of 0.2 mm. Error bars represent one standard deviation. P-values ≤0.05 are indicated, and were calculated using the Student's t-test. (D) Shown are representative images of M9 agar plates of pBR322 (control) or pJRC210 (β+) transformants following incubation for 16 hrs at either 30° or 42°C. Colony diameters in panel D were not measured due to the small size of the pJRC210 transformants (i.e., diameters were <0.2 mm). Each transformation experiment was performed at least 3 independent times; results from one representative experiment are shown.