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. 2014 Jun 4;9(6):e98988. doi: 10.1371/journal.pone.0098988

Figure 3. The transient GUS expression analysis of T-DNA assembled fragment of pSiM24 containing GUS and pSiM24-GUS in onion epidermal cells.

Figure 3

(A) Light microscopy images of X-gluc-treated onion epidermal cells bombarded with pBTRKM24-GUS (T-DNA assembled fragment of pSiM24 with GUS reporter gene) construct DNA-loaded gold particles are presented. Control represents untransformed onion epidermal cells. These micrographs are representative of data collected after examination of onion epidermal cells from a minimum of four independent experiments. Scale bar, 100 µm. (B) Light microscopy images of X-gluc-treated onion epidermal cells bombarded with pSiM24-GUS (pSiM24 with GUS reporter gene) construct DNA-loaded gold particles are presented. Control represents onion epidermal cells with pSiM24 without GUS reporter gene. These micrographs are representative of data collected after examination of onion epidermal cells from a minimum of four independent experiments. Scale bar represents 100 µm.