Table 1. Transformation frequencies of Escherichia coli strain TB1 for pSiM24 binary vector.
| Plasmid | Total size (kb) | Amount DNA used (pg) | Average colony (n>4) | Ratio to pCAMBIA control | Ratio to pKM24KH control |
| pSiM24 | 7.08 | 100 | 634±54b | 4.73 | 5.66 |
| pSiM24-GUS | 8.89 | 125 | 619±46b | 4.62 | 5.53 |
| pSiM24-GFP | 7.8 | 110 | 608±38b | 4.54 | 5.43 |
| pCAMBIA2300 | 8.74 | 123.4 | 134±12a | 1 | 1.2 |
| pKM24KH | 12.94 | 183 | 112±13a | 0.84 | 1 |
The bacteria were transformed with equal molar amounts of each of the plasmid DNA. Statistical analysis of the data was performed adopting one way ANOVA analysis (using GraphPad Prism version 5.01) and presented as the means ± S.D. A P value of less than 0.05 was considered significant indicated by different superscript letters.