Figure 7. Time-course of ATPase activity and formation of trapped Pgp.

(A) Transient behavior of ATPase activity (red) and the fraction of trapped enzyme (blue), evaluating with at the indicated concentration pulses of ATP and V_i. (B) Time-course of the fraction of trapped Pgp according to V_i affinity. Transient behavior of the fraction of trapped enzyme on incubation with ATP and V_i, evaluating with for pulses of 200 µM ATP and V_i of 50 s duration (not shown). Each curve corresponds to  =  3 µM (blue), 0.1 µM (black) and 0.01 µM (red). Values of k are given in Table 2 ; [P]_t = 0.25 µM.