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The NTP constitutively activates PTK activities. Western blot analysis of spleen cell lysates from CON and NTP-treated rats after incubation with anti-αβ-TCR MAb for 0 and 2 min. PTK activity was determined by immunoblotting using antiphosphotyrosine (anti-Pγ) MAb. The positions of molecular mass (MW) markers (in kilodaltons) are shown to the right of the gel.
