Table 2.
Primers used for real-time PCR analysis and normalized Ct values in granulosa cells
| Gene | Primer forward: 5′-3’ | Primer reverse: 5′-3 | Accession number | Average (Δ) Ct ± SEM | Annealing temperature °C | Fragment size (bp) |
|---|---|---|---|---|---|---|
|
ACVR1 |
CCTCTCCTGTGGGAATGAGG |
CTGGAAGCAGCCTTTCTGGT |
XM_001491549.3 |
8.95 ± 0.32 |
60 |
100 |
|
ACVR2B |
ATGTACCGGCATCGAAAACC |
CGAGCCTTGATCTCCAACAG |
XM_001488736.4 |
13.99 ± 0.39 |
60 |
119 |
|
TGFBR2 |
GACCCCAAGCTCACCTACCA |
TGCACTCATCAGAGCTACAGGA |
XM_005600872.1 |
0.8 ± 0.43 |
60 |
124 |
|
CDKN2B |
CCGAGCTGCTACTGCTCCAC |
CACCAGCGTGTCCAGGAAG |
XM_001496235.3 |
13.93 ± 0.70 |
60 |
107 |
|
COL1A2 |
AGGTTTCCAAGGACCTGCTG |
GGTTTTCCAGGGTGACCATC |
XM_005609220.1 |
16.21 ± 0.75 |
60 |
117 |
|
COL3A1 |
GTCCCAACCCAGAGATTCCA |
CGCTACTTTCATTTCCTTTCAGG |
XM_001917620.3 |
2.05 ± 0.57 |
60 |
102 |
|
USF2 |
GGATACCACGGCTGTGTCAG |
ATCGTCCTCTGCGTTCCTGT |
XM_005596152.1 |
4.23 ± 0.70 |
60 |
114 |
|
IL6 |
GGCAGAAAAAGACGGATGCT |
CACCCTTGAACTCGTTCTGGA |
XM_005609172.1 |
13.03 ± 0.38 |
60 |
122 |
|
ITGB7 |
TGCCGAAGGATACCCTGTAGA |
CTGCAGCTTCTCCAGCAAGG |
XM_005611222.1 |
14.58 ± 0.82 |
60 |
112 |
|
TGFB1 |
CTCAGTGCCCACTGCTCCT |
CATCAATGGTGGCCAGATCA |
XM_005596086.1 |
2.68 ± 0.27 |
60 |
100 |
|
ID1 |
ACATGAACGGCTGCTACTCG |
TCCAACTCCAGGTCCCAGAT |
XM_005604549.1 |
10.02 ± 0.51 |
60 |
124 |
|
ID2 |
CATCCCCCAGAACAAGAAGG |
TGGTGATGCAGGCTGACAAT |
XM_001503611.3 |
2.37 ± 0.18 |
60 |
180 |
|
ACTB |
CGACATCCGTAAGGACCTGT |
CAGGGCTGTGATCTCCTTCT |
NM_001081838.1| |
|
60 |
99 |
| GAPDH | AGAAGGAGAAAGGCCCTCAG | GGAAACTGTGGAGGTCAGGA | NM_001163856.1 | 60 | 87 |