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Sensitivity analysis of DNMT3A R882H detection. 1) Endonuclease restriction analysis of serial dilutions of DNMT3A R882H; Undiluted mutation ratio was 59% (estimated by sequencing). Mutated allele wa detected up to a degree of 0.05%. 2) Difference plot for HRM analysis of serial dilutions of DNMT3A R882H: Correct estimation was possible up to a mutation ratio of 5.9%; lower mutation ratios were identified false-negative. Normalisation was performed to the wt allele.
