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. 2014 May 7;4:25. doi: 10.1186/2045-3701-4-25

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Copyright © 2014 Liao et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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PP4 deficiency reduces LPL and IEL αβ T cell numbers without significantly impacting their subset distributions. A-B, LPL (A) and IEL (B) were isolated and analyzed for the expression of various markers by flow cytometry. Results on total (left panels), TCRβ/TCRδ-gated (middle three panels), or B220^-Gr1^--gated (right three panels) cells are shown (n = 2-8). See Additional file 1: Figure S1 for flow cytometry gating strategies.