Table 1.
Cellulose hydrolysis activities of C. thermocellum strains
| C. thermocellum strains | V max (mM glucose equivalent per h/%) | t max (h/fold) |
|---|---|---|
| WT (DSM1313)* |
2.84/100 |
26/1.00 |
| CipA-ΔXDocII |
2.58/91 |
41/1.58 |
| CipA-Δ6CohI |
1.54/54 |
81/3.16 |
| CipA-ΔCBM-1† |
0.31/11 |
115/4.42 |
| CipA-ΔCBM-2† |
0.22/8 |
206/7.92 |
| ΔSdbA |
2.44/86 |
26/1.00 |
| ΔOrf2p |
2.31/81 |
28/1.08 |
| ΔOlpB |
2.19/77 |
40/1.54 |
| Δ7CohII | 2.14/75 | 36/1.38 |
*V max and t max of the WT strain are set as 100% and one fold, respectively. The curve-fitting parameters are listed in Additional file 10.
†The targetron in CipA-ΔCBM-2 inserts at the very end of the second CohI module of CipA, possibly affecting the function of this module and resulting in lower V max and longer t max values compared with CipA-ΔCBM-1.