Table 3.
Helicobacteraceae DNA was detected by nested PCR in colonic biopsies
| Primer a | Sequence (5' - 3') b | Product size (bp) | PCR conditions | Target sequence | References c |
|---|---|---|---|---|---|
| H276f |
CTA TGA CGG GTA TCC GGC |
374 |
94°c for 15 min and 30 s,52°c for 30 s,72°c for 30 s, 35 cycles,72°c for 7 min |
Helicobacter spp.16S rRNA |
Riley et al. [17] |
| H676r |
ATT CCA CCT ACC TCT CCC A |
|
|
|
|
| p17f |
ATG GAA CAG ATA AAG ATT TTA AAG CAA CTT CAG |
435 |
94°c for 15 min and 30 s,55°c for 30 s,72°c for 30 s, 35 cycles,72°c for 7 min |
H. bilis specifict protein |
Feng et al. [21] |
| p17r |
CTA TGC AAG TTG TGC GTT AAG CAT |
|
|
|
|
| Fe1F |
TTT GGT GCT CAC TAA CGC CCT C |
434 (438) |
94°c for 15 min and 30 s,52°c for 30 s,72°c for 30 s, 35 cycles,72°c for 7 min |
H. felis urease gene |
Baele et al. [19] |
| Fe3R |
TTC AAT CTG ATC GCG TAA AG |
|
|
|
|
| Bi1F |
AAC CAA YAG CCC CAG CAG CC |
373 |
94°c for 15 min and 30 s,54°c for 30 s,72°c for 30 s, 35 cycles,72°c for 7 min |
H. bizzozeronii urease gene |
Baele et al. [19] |
| Bi2R |
TGG TTT TAA GGT TCC AGC GC |
|
|
|
|
| HSALF |
CAT TTT CAA AGA GGG CTT GC |
537 |
94°c for 15 min and 30 s,51°c for 30 s,72°c for 30 s, 35 cycles,72°c for 7 min |
H. salomonis HSP60 |
Mikkonen et al. [20] |
| HSALR |
GCA CAC CCC TCA GTT TGT TT |
|
|
|
|
| HP-FOR |
TTA TCG GTA AAG ACA CCA GAA A |
132 |
94°c for 15 min and 30 s,49°c for 30 s,72°c for 30 s, 35 cycles,72°c for 7 min |
H. pylori urease C gene |
He et al. [22] |
| HP-REV |
ATC ACA GCG CAT GTC TTC |
|
|
|
|
| canis3Fd |
TAA GCG CGG TAT GGA TAA GG |
254 |
94°c for 15 min and 30 s,52°c for 30 s,72°c for 30 s, 35 cycles,72°c for 7 min |
H. canis HSP60 |
EU233451 |
| canis4Rd | TTA AGT AGC CGC GGT CAA AC |
a:Different Types of Primers, b:Sequence of Helicobacteraceae DNA, c:References studied for all the selected procedures, d:comparison between numerical continuous of the primer in the helicobacter canis.