Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2014 Jun 4;9(6):e99056. doi: 10.1371/journal.pone.0099056

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2014 Hu et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

PMC Copyright notice

The MTT assay was applied to evaluate the effects of CXCL16-CXCR6 on cell viability in vitro. A: A549 cells from blank-control, shRNA-control and CXCR6-shRNA (2819-1) groups were seeded in 96-well flat-bottom microplates with a density of 2×10³ cells/well. The in vitro viability was observed at 24, 48, 72, 96 and 120 h respectively. B: After starved with 1640 without FCS for 12 h, the cells from blank-control, shRNA-control or CXCR6-shRNA (2819-1) groups were treated with control medium (1640) or CXCL16 at a concentration of 100 ng/mL for 48 h. Then the in vitro viability was recorded by MTT assay. The experiments were repeated three times. Error bars depict the standard error of the mean. ^aP<0.01, compared with the corresponding control.