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(A) Protein structure of E.coli HSP70 (DNAK) chaperone (aa 1–605) complexed with ADP and substrate (PDB: 2KHO). (B) The truncated bovine HSC70 (aa 1–554) mutant E214A/D214B in post-ATP bound phase (PDB: 1YUW). The truncated form is missing a portion of the C-terminal α-helices, but retains the ATPase site within the nucleotide binding domain.
