Skip to main content
. 2014 Jun 4;9(6):e99049. doi: 10.1371/journal.pone.0099049

Figure 3. Endogenous Ets1 plays a role in TXNIP expression in Min6 cells.

Figure 3

(A). Ets1 siRNA 2, 5, 6 and 7 silenced endogenous Ets1 protein in Min6 cells. Min6 cells were cultured in DMEM containing 25 mM of glucose. Scrambled siRNA and commercially designed siRNAs 2, 5, 6, 7 against mouse Ets1 were transfected into Min6 cells. After 48 hrs, the cell lysates were prepared and subjected to Western blot analysis with Ets1 antibody. (B). Knocking down of Ets1 resulted in reduced expression of TXNIP protein in Min6 cells as determined by Western blot. The cell lysates same as (A) were subjected to Western blot analysis with TXNIP antibody. (C). Inhibition of ERK1/2 by 20 µM of PD98059 blocked TXNIP expression in Min6 cells as determined by Western blot. n = 3.