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. 2014 Jun 4;9(6):e99049. doi: 10.1371/journal.pone.0099049

Figure 4. Ets1 is associated with txnip gene promoter and activates txnip promoter reporter with p300.

Figure 4

(A). Endogenous Ets1 is associated with txnip promoter region (-400 bp) as shown by chromatin IP analysis in Min6 cells. RNA polymerase II antibody (shown as RNA Poly ab) was used as positive control and IgG was used as negative control. One percent of Input chromatin was also shown. (B). Ets1 and p300 activate txnip promoter luciferase reporter. HEK293 cells were transiently transfected with txnip proximal promoter (1 kb) luciferase reporter in the presence or absence of Ets1 and/or p300. (n = 3; values are shown as mean ± SD. *p<0.05, **p<0.01, ***p<0.001. N.S., not significant, t-test).