Table 1.

Primers used

Gene	Forward primer (5′ to 3′)	Reverse primer (5′ to 3′)	Annealing temp. °C	Product size (bp)
speA1-3,6 [15]	5′-GGACTAACAATCTCGCAAGAGG-3′	5′-TTACTTGGTTGTTAGGTAGACTTC-3′	54	696
speA4,5 [15]	5′-GCTAACAACCTCACAAGAAG -3′	5′-TGCTTGAGTCAAGCGTCTC-3′	53	659
speC[15]	5′-GATTTCTACTATTTCACC-3′	5′-AAATATCTGATCTAGTCCC-3′	44	584
speG[15]	5′-CTTATGCAGATGAAAATTTAAAAGATT-3′	5′-AAAGCAAAGGGGGGAGAATAG-3′	53.2	664
speI[15]	5′-ATGAGTAGTGTGGGAGTTATTAA-3′	5′-ATGAAGTTGATCAGAATAAGCG-3′	55	645
speJ[15]	5′-ATCTTATTTAGTCCAAAGGTAAAT-3′	5′-GTGAACGAGGAGAGGTATGAA-3′	55.5	718
smeZ[15]	5′-TTGGATTAGAAGTAGATAA-3′	5′-TTGAATTAGGAGTCAATTTCTATATCT-3′	52	638
ssa[15]	5′-AGTCAGCCTGACCCTACTCCA-3′	5′-TAAGGTGAACCTCTATAGCTATAG-3′	59.1	691
speH[6]	5′-TTGGATCCAATTCTTATAATACAACC-3′	5′-CCACTTCCTGAGCGGTTACTTTCGG-3′	50	399
speJ[6]	5′-CTATGGTGGAATTACACC-3′	5′-CATGTTTATTGCCATTGATCGC-3′	50	247
speK[6]	5′-GCGGATCCGATACGTACAATACAAATG-3′	5′-GCGAATTCAATAGCATTCAACCA-3′	50	798
speL[6]	5′-GCAGCCATATGGAAGAGACTATTCCTCTTAAGGATATATACTCTC-3′	5′-GGGGATCCTTAATTTTCTTTGTTTGTGAATAAATAGAC-3′	50	703
speM[6]	5′-GCAGCCATATGTTTTCAGATGCTGTGTTGG-3′	5′-GGGGATCCTTAATTTTTAGAAAAATCTTCGTTTAAGTA-3′	50	661
speB[6]	5′-GGATCCCAACCAGTTGTTAAATCTCT-3′	5′-AACGTTCTAAGGTTTGATGCCTACAA-3′	50	774

All primer sequences shown here have previously been published by Maripuu et al. or Commons et al.