TABLE 1.
Discrepant quantitative results for detection of gB and pol genes in a duplex qPCR assay for CMV
Patient identification | Day | Result for gB (copies/ml) | Result for pol (copies/ml) | Ratio of pol/gB |
---|---|---|---|---|
A | 1 | 0 | 0 | |
8 | 0 | 0 | ||
15 | 40 | 800 | 20 | |
22 | 200 | 4,600 | 23 | |
29 | 3,800a | 191,000 | 50 | |
37 | 7,000 | 370,000 | 53 | |
40 | 34,000 | 1,200,000 | 35 | |
44 | 62,000 | 2,100,000 | 34 | |
51 | 35,000 | 200,000 | 5.7 | |
58 | 1,300 | 10,600 | 8.1 | |
61 | 5,400 | 10,000 | 1.8 | |
65 | 4,000 | 34,600 | 8.6 | |
72 | 4,400 | 20,000 | 4.5 | |
79 | 800 | 13,000 | 16 | |
87 | 2,000 | 12,800 | 6.4 | |
93 | 3,000 | 19,000 | 6.3 | |
96 | 1,200 | 13,000 | 10 | |
110 | 240 | 7,900 | 33 | |
115 | 3,800 | 37,400 | 10 | |
117 | 2,500 | 35,400 | 14 | |
122 | 200 | 1,800 | 9.0 | |
128 | 80 | 700 | 8.8 | |
139 | 16,000 | 300,000 | 19 | |
142 | 12,400a | 280,000 | 23 | |
B | 1 | 0 | 0 | |
7 | 0 | 200 | ||
15 | 80 | 200 | 2.5 | |
21 | 340 | 2,100 | 6.2 | |
28 | 900a | 5,200 | 5.8 | |
35 | 2,300 | 8,400 | 3.7 | |
42 | 360 | 640 | 1.8 | |
47 | 0 | 800 | ||
53 | 880 | 5,000 | 5.7 | |
56 | 1,400a | 6,800 | 4.9 |
Sequence analysis was performed on this sample.