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. 2014 May 16;11:91. doi: 10.1186/1742-2094-11-91

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Copyright © 2014 Ashraf et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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ELISA analysis of (A) IL-1β and (B) TNF-α secretion in the CSF of gp120 (500 ng) administered rats receiving simultaneous intraperitoneal injection of chloroquine or minocycline or simvastatin. CSF collected from saline-injected animals was used as a control. Results are expressed as mean ± SEM. Samples obtained from at least 10 different animals were used per group. Asterisks represent data points significantly different from saline-administered animals (***P <0.001; **P <0.01). Diamonds represent data points significantly different from gp120 treatment (♦♦♦P <0.001). (ELISA = Enzyme linked immunoabsorbant assay; IL-1β = interleukin 1β; TNFα = tumor necrosis factorα; CSF = cerebrospinal fluid; gp120 = glycoprotein120; CQ = chloroquine; Mino = minocycline; Sim = simvastatin).