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. 2014 May 7;7:50. doi: 10.1186/1757-2215-7-50

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Copyright © 2014 Douville and Sirard; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver ( http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Histogram of modeled DNA content for follicles 6-9 mm in diameter at different growing phases. The modelization of the growing, plateau and atretic phases, using FlowJo software, gave the proportion of total events in the sub-G1 phase (-G1), corresponding to cells with less than 2n DNA content, G1 phase, corresponding to cells containing 2n of DNA, S phase, corresponding to cells that are duplicating their DNA material, G2 phase, corresponding to cells having completely duplicated their genetic material to 4n. (n = 26 granulosa samples). On the graph, the selected regions correspond to 1) –G1, 2) G0/G1, 3) S.