FIG. 4.
Comparison of dynamic ranges of N-gene- and 1b-gene-specific PCRs. The dynamic ranges of both N-gene- and 1b-gene-specific PCRs were obtained with the same plasmid construct into which a 1:1 ratio of the corresponding amplicons were subcloned. The serially diluted plasmid, with copy numbers ranging from 10−1 to 105 copies, was used as a template for both PCRs. Triangles, N-gene-specific PCR; gray lines, 1b-gene-specific PCR. The inset shows CT values for a triplicate set of experiments of both PCRs with different copy numbers of the template. NTC, no-template control. The x axis indicates the cycle number of the quantitative PCR, while the y axis represents the fluorescence.