FIG. 6.

Northern blot analysis of SARS-CoV total RNA. The total RNA of SARS-CoV was extracted from SARS-CoV-infected Vero E6 cells. The RNA was separated in a 1% denaturing gel containing 6.29% formaldehyde. Afterwards, the RNA was transferred to a positively charged nylon membrane and hybridized with digoxigenin-labeled PCR fragments specific for the 1b, S, M, and N genes. Lane 1, 1b; lane 2, S; lane 3, M; lane 4, N. The vertical bar shows the molecular size reference. Arrows indicated the transcripts hybridized with the N probe. The signals were analyzed by chemiluminescence.