Figure 2. NAD(P)H fluorescence decay responses to metabolic perturbation are mechanism dependent.

(a–d) Time series of NAD(P)H fluorescence intensity following treatments chosen to perturb oxidative (a: respiratory chain inhibition by rotenone, b: uncoupling with FCCP) and glycolytic metabolism (glucose replaced by deoxyglucose with c: pyruvate or d: lactate supplied as substrate). (e) Colour-coded images and (f,g) quantification of changes in τ_bound and α_bound on application of treatment. Scale bar, 20 μm. Error bars indicate±s.d., *P<0.05 (two-tailed Student’s t-test, n=9).