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. 2014 Jun 5;4:5183. doi: 10.1038/srep05183

Figure 5. Tendons from CLOCKΔ19 mice exhibit ectopic calcification.

Figure 5

(A) Expression of Grem2 in tail tendons of CLOCKΔ19 mice compared with wild type tendons at CT7 (peak expression) and CT19 (nadir expression) (n = 3). qPCR values normalized to GAPDH. *p < 0.05, one-way ANOVA. Error bars indicate the SEM. (B) Protein was purified from CLOCKΔ19 tail tendons at CT7 and CT19 and phosphorylation of Smad1/5 were analyzed by western blotting (n = 2). Western blot analysis of GAPDH protein served as a loading control. (C) Longitudinal sections of Achilles tendon from 18 week-old wild type and CLOCKΔ19 mice stained with Alizarin red. b = attachment of tendon to bone, m = attachment of tendon to muscle. Bars = 500 µm.