Figure 2. Pan-neuronal and TRN-specific re-expression of PTL-1 rescues the neuronal ageing phenotype in TRNs that is observed in the ptl-1(ok621) null mutant.

The TRNs were visualised using the Pmec-4::gfp (zdIs5) reporter. The presence of the ptl-1(ok621) mutation in the genetic background of each transgenic line is indicated by the addition of “ptl-1(ok621)” in the strain name. (a) Representative images of a young TRN, and TRNs showing cell body branching (arrow) or axon blebbing. (b) Neuron imaging assay conducted for pan-neuronal transgenic worms (“Pan-neuronal Tg”) showing (i) cell body branching and (ii) axon blebbing. (c) Neuron imaging assay conducted for TRN-specific transgenic worms (“TRN Tg”). Data for wild-type and ptl-1(ok621) animals in both graphs were obtained in the same experiment. n for each time-point is indicated under the graphs. The chi-squared statistical test was used to determine statistical significance. P-value is indicated by ns = not significant, *<0.05. Details of p-values are shown in Supplementary Table S1. Experiments were conducted twice independently, and the representative data shown are from one experiment.