Figure 3. Pan-neuronal but not TRN-specific re-expression of PTL-1, rescues the neuronal ageing phenotype in GABAergic neurons that is observed in the ptl-1(ok621) null mutant.

The GABAergic neurons were visualised using the Punc-47::gfp (oxIs12) reporter. The presence of the ptl-1(ok621) mutation in the genetic background of each transgenic line is indicated by the addition of “ptl-1(ok621)” in the strain name. (a) Representative images of young or branched GABAergic neurons. (b) Neuron imaging assay conducted for “Pan-neuronal Tg” worms. (c) Neuron imaging assay conducted for “TRN Tg” worms. Data for wild-type and ptl-1(ok621) animals in both graphs were obtained in the same experiment. n for each time-point is indicated under the graphs. The chi-squared statistical test was used to determine statistical significance. P-value is indicated by ns = not significant, *<0.05. Details of p-values are shown in Supplementary Table S1. Experiments were conducted twice independently, and the representative data shown are from one experiment.