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. 2014 May 29;13:129. doi: 10.1186/1476-4598-13-129

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Copyright © 2014 Felder et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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OC125 weakly binds to cells that are generally considered negative for MUC16. SKOV-3 cells (identity confirmed by STR analysis) were stained with the primary (1°) antibodies OC125 or VK8 followed by incubation with allophycocyanine (APC)-labeled donkey anti-mouse (DAM) secondary antibody. Binding of the antibodies to SKOV-3 cells was determined on a LSR-II flow cytometer. RT-PCR using MUC16 primers previously reported [178], was used to determine expression of MUC16 in OVCAR-3, ECC-1 and SKOV-3 cells (data not shown).