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. 2014 May 29;13:129. doi: 10.1186/1476-4598-13-129

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Copyright © 2014 Felder et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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MUC16 on the surface of ovarian cancer cells. OVCAR-3 cells were labeled with VK8 followed by colloidal gold nanoparticles conjugated with goat anti-mouse secondary antibody. A, low magnification secondary electron image of two labeled OVCAR-3 cells. B, Scanning Electron Microscopy (SEM) image of OVCAR-3 showing colloidal gold nanoparticles binding to cell surface and microvilli. C, Back scattered electron image of same cell surface shown in B, clearly showing the colloidal gold nanoparticles. Bright spots (some indicated by bright arrows) in B and C are the colloidal gold nanoparticles. OVCAR-3 cells are not labeled with colloidal gold nanoparticles in the absence of VK-8 (data not shown).