Figure 1.

Effect of CYP27A1 deficiency on the development of atherosclerosis: en face analysis. ApoE-KO, het, and DKO mice were fed for 3 or 6 mo with a CD or WD before they were euthanized, and aorta was analyzed en face. A, B) Quantification of atherosclerotic lesions after 3 or 6 mo of CD (A) or WD (B). Data are means ± sem (n=4–6). *P < 0.05, **P < 0.01, ***P < 0.001. C) Representative en face Sudan IV staining of sections of aortas of mice fed the WD for 3 mo. Scale bars = 1 mm. ND, not done.