Figure 3.

Effect of CYP27A1 deficiency on macrophage accumulation in atherosclerotic lesions. ApoE-KO, het, and DKO mice were fed for 3 to 6 mo with a CD or WD. Macrophages were stained with anti- MAC-1 antibody. A, B) Quantification of abundance of macrophages in atherosclerotic lesions after 3 or 6 mo of CD (A) or WD (B). *P < 0.05, **P < 0.01, ***P < 0.001. Data are means ± sem (n=4). C) Representative photomicrographs of aortic root in paraffin-embedded sections stained with anti-MAC-1 antibody in mice fed the WD for 3 mo. Arrows indicate the cholesterol cleft. Scale bars = 20 μm. ND, not done.