Table 2.
Effect of CYP27A1 expression on plasma, liver, and bile biochemistry and on bile acid concentration in ApoE-KO, het, and DKO mice fed a CD or WD for 3 mo
| Parameter | ApoE-KO |
Het |
DKO |
P | |||
|---|---|---|---|---|---|---|---|
| CD | WD | CD | WD | CD | WD | ||
| Plasma | |||||||
| ALT (U/L) | 57 ± 50 | 32 ± 12 | 59 ± 53 | 37 ± 25 | 88 ± 57 | 85 ± 40 | NS |
| AST (U/L) | 208 ± 103 | 66 ± 75* | 257 ± 171 | 123 ± 84* | 506 ± 374 | 325 ± 235## | <0.0002 |
| Glucose (mM) | 8.2 ± 3.6 | 8.4 ± 1.8 | 8.6 ± 1.9 | 9.1 ± 3.1 | 10.5 ± 4.4 | 8.9 ± 2.2 | NS |
| Bilirubin (μM) | 1.0 ± 1.6 | 24.8 ± 20** | 2.9 ± 1.6# | 21.8 ± 1.8** | <0.1 | 0.3 ± 1.0## | <0.0001 |
| Bile acids (μg/ml) | 9.8 ± 12.4 | 2.5 ± 1.0 | 6.3 ± 8.7 | 2.5 ± 1.8 | 4.4 ± 1.7 | 4.7 ± 2.4 | NS |
| Liver | |||||||
| TG (nmol/mg) | 39 ± 11 | 55 ± 23 | 57 ± 25 | 56 ± 23 | 78 ± 20# | 65 ± 21 | <0.02 |
| TC (mmol/mg) | 4.4 ± 0.7 | 5.6 ± 1.9 | 4.4 ± 0.6 | 5.9 ± 1.6 | 3.8 ± 0.5 | 4.2 ± 0.6 | <0.0189 |
| CE (μg/mg) | 0.9 ± 0.5 | 0.4 ± 0.2 | 0.5 ± 0.4 | 0.4 ± 0.2 | 0.3 ± 0.3 | 0.3 ± 0.2 | <0.0483 |
| Bile acids (μg/g) | 7.4 ± 3.7 | 45.9 ± 45.6 | 17.8 ± 10.9 | 26.7 ± 16.6 | 5.9 ± 3.6 | 6.2 ± 2.4* | <0.0045 |
| Bile | |||||||
| TC (M) | 2.8 ± 1.2 | 4.0 ± 2.5 | 3.2 ± 0.7 | 4.0 ± 1.9 | 2.0 ± 0.7 | 1.8 ± 0.9 | NS |
| Bile acids (μg/μl) | 1.6 ± 1.3 | 1.3 ± 1.4 | 1.8 ± 2.3 | 0.6 ± 0.4 | 0.3 ± 0.0.5## | 0.3 ± 0.02## | <0.0008 |
| Urine bile acids (μg/μl) | 18.7 ± 11.2 | 11.0 ± 18.0 | 10.4 ± 10.3 | 14.2 ± 7.2 | 8.2 ± 7.7 | 1.0 ± 0.5## | <0.0001 |
Results are presented as means ± sd (n=4–14).
*
P < 0.01,
**
P < 0.001 vs. CD;
#
P < 0.01,
##
P < 0.001 vs. ApoE-KO.