Figure 5.

Mitotic and apoptotic markers are differentially expressed in PCa cell lines after treatment with BI 2536 and BI 6727. A) Lysates from PCa cells treated with BI compounds were probed for various mitotic markers. DU145 and LNCaP cells demonstrated elevated levels of phosphorylated histone H3 and cyclin B1, and a decrease in cdc20 levels, indicating an increased cell population in early mitosis. A lack of phosphorylated histone H3 induction and cdc20 down-regulation was evident in PC3 lysates. While cyclin B1 levels did not change after BI 2536 treatment in PC3 cells, an increase was evident at high nanomolar concentration of BI 6727. B) Western blot analyses for cleaved PARP in PCa cells after 24 h of treatment with BI compounds. PARP cleavage was increased in all PCa cell lines after treatment. The numbers above Western blots indicate densitometric quantification of protein expression levels relative to untreated controls, normalized to the housekeeper vinculin. All Western blots are representative of ≥3 independent experiments.