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. 2013 Oct;27(10):4279–4293. doi: 10.1096/fj.12-222893

Figure 7.

Figure 7.

Combination treatment of Plk1 inhibitors BI 2536 and BI 6727 with HDAC inhibitors VPA and SAHA in PCa cell lines has a synergistic effect in both MTS (A) and clonogenic (B) assays after 48 h of treatment. The antitumor effect was most apparent at low concentrations of the agents, in BI 2536 compared to BI 6727, and in SAHA compared to VPA. +, synergy; ++, strong synergy; +++, very strong synergy, as determined by Calcusyn. For DU145 cells: BI 2536 lo, 10 nM; BI 2536 hi, 100 nM (MTS), or 250 nM (clonogenics); BI 6727 lo, 10 nM; BI 6727 hi, 25 nM. For LNCaP cells: BI lo, 50 nM; BI hi, 250 nM. For PC3 cells: BI 2536 lo, 50 nM; BI 2536 hi, 250 nM; BI 6727 lo, 300 nM; BI 6727 hi, 500 nM. Clonogenic and MTS data represent ≥3 independent experiments.