Figure 6.

Rab14 expression in normal rat kidney. A) Immunohistochemistry. Normal rat kidney paraffin sections were dewaxed, hydrated, and then incubated with primary antibodies Rab14 or UT-A1, followed by secondary antibody and avidin-biotin peroxidase complex. Colors were developed by AEC and examined under a microscope. B) Lipid raft experiment. Rat IMCD suspensions were prepared and lysed in TENV buffer. The lysate supernatants were loaded on a 5–40% sucrose gradient as described in Materials and Methods. After 16 h ultracentrifugation, equal amounts of fractions were collected and used for immunoblotting with relevant antibodies.