Figure 3.

Daughter protrusions, not mother protrusions, regulate 3D cell speed through the Arp2/3, N-WASP, cortactin, and Cdc42 module. A, B) Total number of mother protrusions produced per hour (A) and degree of branching from mother protrusions (number of first-generation protrusions per mother protrusion; B) in FAK-, talin-, p130Cas-, and VASP-depleted cells. For all panels, cells were monitored for 16.5 h. For each condition, n = 3; ≥60 cells were probed for protrusion analysis. **P < 0.01, ***P < 0.001 vs. WT. C–F) Assessment of correlation between 3D cell speeds evaluated at time lags of 16 min (C, E) and 1 h (D, F) and the rates of formation of daughter protrusions (C, D) and mother protrusions (E, F).